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Pasteur Institute sk n sh d cell line

A-C : Micrograph of SK-N-SH D cells maintained in: proliferation media for one day (A), differentiation media for two days (B) and differentiation media with MLS-0038949 (25 µM) for two days (C). D. TNAP is involved in the differentiation of SK-N-SH D cells into neurons. Cells were considered differentiated when they bore at least one neurite whose length was twice the cell body diameter. The number of neurites was then normalized by the number of cells in each micrograph . Each dot in the scattergram corresponds to the number of neurites per cell determined for one micrograph. Forty micrographs have been analyzed for each day and condition. The micrographs taken on day 2 were not taken on the same cells and neurites as those of day 1. The central horizontal line corresponds to the median of the number of neurites per cell and the upper and lower bars encompass the 95% confidence interval of the median. The total number of cells that have been classified as either differentiated or not differentiated was: day 1, control: 1398; day 1, MLS-0038949 25 µM: 1689; day 2, control: 2157; day 2, MLS-0038949 25 µM: 2257. Two-way ANOVA and Holm-Šídák’s post hoc tests revealed a strong effect of the TNAP inhibitor. ****: P < 0.0001. E. Distribution of neurite length of differentiated cells measured after one or two days in differentiation medium in the presence or absence of MLS-0038949 (25 µM). The length of the neurites of differentiated cells was not affected by TNAP inhibition. Overall, neurite length has been measured for 4124 differentiated cells in 4 × 40 micrographs (day 1, control: 1017 neurons; day 1, MLS-0038949: 824 neurons; day 2, control: 1379 neurons; day 2, MLS-0038949: 904 neurons).

Sk N Sh D Cell Line, supplied by Pasteur Institute, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews

sk n sh d cell line - by Bioz Stars, 2026-09

86/100 stars

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1) Product Images from "Tissue-nonspecific alkaline phosphatase promotes neuronal cell proliferation and differentiation: metabolomic reveals glutathione and taurine as molecular correlates"

Article Title: Tissue-nonspecific alkaline phosphatase promotes neuronal cell proliferation and differentiation: metabolomic reveals glutathione and taurine as molecular correlates

Journal: bioRxiv

doi: 10.64898/2026.02.24.707745

Figure Legend Snippet: A-C : Micrograph of SK-N-SH D cells maintained in: proliferation media for one day (A), differentiation media for two days (B) and differentiation media with MLS-0038949 (25 µM) for two days (C). D. TNAP is involved in the differentiation of SK-N-SH D cells into neurons. Cells were considered differentiated when they bore at least one neurite whose length was twice the cell body diameter. The number of neurites was then normalized by the number of cells in each micrograph . Each dot in the scattergram corresponds to the number of neurites per cell determined for one micrograph. Forty micrographs have been analyzed for each day and condition. The micrographs taken on day 2 were not taken on the same cells and neurites as those of day 1. The central horizontal line corresponds to the median of the number of neurites per cell and the upper and lower bars encompass the 95% confidence interval of the median. The total number of cells that have been classified as either differentiated or not differentiated was: day 1, control: 1398; day 1, MLS-0038949 25 µM: 1689; day 2, control: 2157; day 2, MLS-0038949 25 µM: 2257. Two-way ANOVA and Holm-Šídák’s post hoc tests revealed a strong effect of the TNAP inhibitor. ****: P < 0.0001. E. Distribution of neurite length of differentiated cells measured after one or two days in differentiation medium in the presence or absence of MLS-0038949 (25 µM). The length of the neurites of differentiated cells was not affected by TNAP inhibition. Overall, neurite length has been measured for 4124 differentiated cells in 4 × 40 micrographs (day 1, control: 1017 neurons; day 1, MLS-0038949: 824 neurons; day 2, control: 1379 neurons; day 2, MLS-0038949: 904 neurons).

Techniques Used: Control, Inhibition

A. 1 H-NMR spectrum of the aqueous extract from an SK-N-SH D untreated cell culture after two days in differentiation medium. The zoomed-in sections, highlighted in colored dashed boxes or pointed at by arrows, correspond to the signals of the metabolites showing the most significant variations (green dots in B and metabolites in C-F): proline (Pro, green box), UMP (orange box), fumarate (Fum, cyan box), methionine (Met, red box), oxidized and reduced glutathione (GSH and GSSG, purple box), glycerophosphocholine (GPC), glycine (Gly, brown box), hypotaurine and taurine (Hyp and Tau), acetate (Ace), and valine, isoleucine and leucine (Val, Ile and Leu, blue box) . B. Ratios of the concentrations expressed as percentages for 31 of the 34 metabolites identified in aqueous extracts of SK-N-SH D cells cultured for two days in differentiation medium in the presence of MLS-0038949 vs . control (not shown: hypotaurine, taurine and glutathione which are detailed in panels D-E) (n = 5 pairs of cultures) . Symbols correspond to the mean ratio and bars encompass the 95% confidence interval of the mean. Green symbols correspond to metabolites whose amounts changed significantly in the presence of MLS-0038949 (paired t-test, P < 0.05), yellow symbols to marginally significant differences (0.05 ≤ P ≤ 0.06) and gray symbols to no significant changes. PC: phosphocholine. C-F. Focus on changes in amounts for organosulfur compounds. Each dot in scattergrams on the left corresponds to one culture. Dots joined by lines correspond to pairs of cultures that were seeded at the same time in proliferation medium with (red dots) and without (black dots) MLS-0038949 (n = 5 pairs of cultures). Right scattergrams: ratio of concentration in MLS vs. control (mean and 95% confidence interval indicated by central and flanking lines). The level of methionine (C), hypotaurine (D) and taurine (E) in-creased significantly while that of glutathione (F) decreased in the presence of MLS-0038949. Significance levels of paired t-tests are given by the number of asterisks: *: P < 0.05; **: P < 0.01. The probability of making a type 1 error being set at P = 0.05, either one or two of the significant differences reported here would be a false positive (0.05 × 34 = 1.7). Yet significant differences were observed in a much larger number of comparisons (13/34 comparisons).

Figure Legend Snippet: A. 1 H-NMR spectrum of the aqueous extract from an SK-N-SH D untreated cell culture after two days in differentiation medium. The zoomed-in sections, highlighted in colored dashed boxes or pointed at by arrows, correspond to the signals of the metabolites showing the most significant variations (green dots in B and metabolites in C-F): proline (Pro, green box), UMP (orange box), fumarate (Fum, cyan box), methionine (Met, red box), oxidized and reduced glutathione (GSH and GSSG, purple box), glycerophosphocholine (GPC), glycine (Gly, brown box), hypotaurine and taurine (Hyp and Tau), acetate (Ace), and valine, isoleucine and leucine (Val, Ile and Leu, blue box) . B. Ratios of the concentrations expressed as percentages for 31 of the 34 metabolites identified in aqueous extracts of SK-N-SH D cells cultured for two days in differentiation medium in the presence of MLS-0038949 vs . control (not shown: hypotaurine, taurine and glutathione which are detailed in panels D-E) (n = 5 pairs of cultures) . Symbols correspond to the mean ratio and bars encompass the 95% confidence interval of the mean. Green symbols correspond to metabolites whose amounts changed significantly in the presence of MLS-0038949 (paired t-test, P < 0.05), yellow symbols to marginally significant differences (0.05 ≤ P ≤ 0.06) and gray symbols to no significant changes. PC: phosphocholine. C-F. Focus on changes in amounts for organosulfur compounds. Each dot in scattergrams on the left corresponds to one culture. Dots joined by lines correspond to pairs of cultures that were seeded at the same time in proliferation medium with (red dots) and without (black dots) MLS-0038949 (n = 5 pairs of cultures). Right scattergrams: ratio of concentration in MLS vs. control (mean and 95% confidence interval indicated by central and flanking lines). The level of methionine (C), hypotaurine (D) and taurine (E) in-creased significantly while that of glutathione (F) decreased in the presence of MLS-0038949. Significance levels of paired t-tests are given by the number of asterisks: *: P < 0.05; **: P < 0.01. The probability of making a type 1 error being set at P = 0.05, either one or two of the significant differences reported here would be a false positive (0.05 × 34 = 1.7). Yet significant differences were observed in a much larger number of comparisons (13/34 comparisons).

Techniques Used: Cell Culture, Control, Concentration Assay

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Journal: bioRxiv

Article Title: Tissue-nonspecific alkaline phosphatase promotes neuronal cell proliferation and differentiation: metabolomic reveals glutathione and taurine as molecular correlates

doi: 10.64898/2026.02.24.707745

Figure Lengend Snippet: A-C : Micrograph of SK-N-SH D cells maintained in: proliferation media for one day (A), differentiation media for two days (B) and differentiation media with MLS-0038949 (25 µM) for two days (C). D. TNAP is involved in the differentiation of SK-N-SH D cells into neurons. Cells were considered differentiated when they bore at least one neurite whose length was twice the cell body diameter. The number of neurites was then normalized by the number of cells in each micrograph . Each dot in the scattergram corresponds to the number of neurites per cell determined for one micrograph. Forty micrographs have been analyzed for each day and condition. The micrographs taken on day 2 were not taken on the same cells and neurites as those of day 1. The central horizontal line corresponds to the median of the number of neurites per cell and the upper and lower bars encompass the 95% confidence interval of the median. The total number of cells that have been classified as either differentiated or not differentiated was: day 1, control: 1398; day 1, MLS-0038949 25 µM: 1689; day 2, control: 2157; day 2, MLS-0038949 25 µM: 2257. Two-way ANOVA and Holm-Šídák’s post hoc tests revealed a strong effect of the TNAP inhibitor. ****: P < 0.0001. E. Distribution of neurite length of differentiated cells measured after one or two days in differentiation medium in the presence or absence of MLS-0038949 (25 µM). The length of the neurites of differentiated cells was not affected by TNAP inhibition. Overall, neurite length has been measured for 4124 differentiated cells in 4 × 40 micrographs (day 1, control: 1017 neurons; day 1, MLS-0038949: 824 neurons; day 2, control: 1379 neurons; day 2, MLS-0038949: 904 neurons).

Article Snippet: The SK-N-SH D cell line (RRID: CVCL_B0GN; Pasteur Institute, Collection Nationale de Cultures de Micro-organismes I-5010; ) is a subclone of the SK-N-SH (RRID: CVCL_0531) human neuroblastoma cell line ( ).

Techniques: Control, Inhibition

Journal: bioRxiv

Article Title: Tissue-nonspecific alkaline phosphatase promotes neuronal cell proliferation and differentiation: metabolomic reveals glutathione and taurine as molecular correlates

doi: 10.64898/2026.02.24.707745

Figure Lengend Snippet: A. 1 H-NMR spectrum of the aqueous extract from an SK-N-SH D untreated cell culture after two days in differentiation medium. The zoomed-in sections, highlighted in colored dashed boxes or pointed at by arrows, correspond to the signals of the metabolites showing the most significant variations (green dots in B and metabolites in C-F): proline (Pro, green box), UMP (orange box), fumarate (Fum, cyan box), methionine (Met, red box), oxidized and reduced glutathione (GSH and GSSG, purple box), glycerophosphocholine (GPC), glycine (Gly, brown box), hypotaurine and taurine (Hyp and Tau), acetate (Ace), and valine, isoleucine and leucine (Val, Ile and Leu, blue box) . B. Ratios of the concentrations expressed as percentages for 31 of the 34 metabolites identified in aqueous extracts of SK-N-SH D cells cultured for two days in differentiation medium in the presence of MLS-0038949 vs . control (not shown: hypotaurine, taurine and glutathione which are detailed in panels D-E) (n = 5 pairs of cultures) . Symbols correspond to the mean ratio and bars encompass the 95% confidence interval of the mean. Green symbols correspond to metabolites whose amounts changed significantly in the presence of MLS-0038949 (paired t-test, P < 0.05), yellow symbols to marginally significant differences (0.05 ≤ P ≤ 0.06) and gray symbols to no significant changes. PC: phosphocholine. C-F. Focus on changes in amounts for organosulfur compounds. Each dot in scattergrams on the left corresponds to one culture. Dots joined by lines correspond to pairs of cultures that were seeded at the same time in proliferation medium with (red dots) and without (black dots) MLS-0038949 (n = 5 pairs of cultures). Right scattergrams: ratio of concentration in MLS vs. control (mean and 95% confidence interval indicated by central and flanking lines). The level of methionine (C), hypotaurine (D) and taurine (E) in-creased significantly while that of glutathione (F) decreased in the presence of MLS-0038949. Significance levels of paired t-tests are given by the number of asterisks: *: P < 0.05; **: P < 0.01. The probability of making a type 1 error being set at P = 0.05, either one or two of the significant differences reported here would be a false positive (0.05 × 34 = 1.7). Yet significant differences were observed in a much larger number of comparisons (13/34 comparisons).

Techniques: Cell Culture, Control, Concentration Assay

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